Разработка методики экстракции левамизола из биологических жидкостей

З.У. Усманалиева , К.С. Ризаев , Д.А. Зулфикариева , М.О. Рохаталиева

Abstract

Aim. In cases of acute poisoning, in order to provide emergency medical care to the patient, it is necessary
to correctly determine the cause of the poisoning. When analyzing blood and urine for the identification
of a poisonous substance in a forensic chemical examination, it is important to extract this substance from
biological fluids. The aim of this work was to develop isolation conditions using the extraction of levamisole
from biological fluids of laboratory animals.
Material and methods. To develop optimal conditions for isolating levamisole from biological fluids of
laboratory animals, levamisole was first extracted from an aqueous medium under different conditions. Several
solvents were used to study the effect of the nature of the organic solvent on the separation process. The
influence of the pH of the medium on the extraction of levamisole from the aqueous medium was also studied.
Results. According to the results of the analysis, it was found expedient to carry out three-fold extraction
of levamisole from the aqueous medium with chloroform at a pH of 3-4. This technique was used to isolate
levamisole from blood and urine of laboratory animals.