ANALYSIS OF MORPHOMETRIC CHARACTERISTICS OF VARIOUS HIPPOCAMPAL ZONES AFTER SURGERY USING DIFFERENT TYPES OF ANESTHESIA

V.E. KOCHETOV, V.H. SHARIPOVA, A.A. VALIHANOV, A.H. ALIMOV

Abstract

Material and methods. An experimental research was performed on 50 white male rats aged 8 months weighing 254 - 302 g. All animals were divided into 3 groups: 1 control and 2 experimental. The experimental groups were divided depending on the type of general anesthesia (3 subgroups) and the type of surgery (6 subgroups). Isoflurane, ketamine or propofol was used as anesthetics in combination with fentanyl. Minor surgery was simulated by a median laparotomy alone, and major ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ     48Вестник экстренной медицины, 2020, том 13, No6surgery – by one-side nephrectomy. The histological preparations of the rat brain were studied in light and fluorescent microscopes.Results. During histological and morphometric studies, pathologically altered – hyperchromic, deformed, reduced or enlarged neurons were found in all experimental groups. The number of damaged neurons and their sizes were different in different subgroups and hippocampal zones. No regularity of these changes was observed. The greatest damage to neurons was found in rats after minor surgery using propofol anesthesia and major surgery group where isoflurane was used for anesthesia. The smallest changes in neurons were observed in rats after minor surgery using isoflurane anesthesia, and after major surgery using propofol anesthesia.Conclusion. General anesthesia and surgery can damage the neurons of the pyramidal layer of the rat hippocampus. No correlation was found between the type of general anesthesia and the invasivenessof surgery.